(…) developed a multi-tiered analytical framework, integrating in-vitro digestion models, high-resolution electron microscopy, and biochemical quantification techniques, precisely replicating salivary and gastric physiological environments to evaluate encapsulation stability and autonomous bioactive release dynamics. Through INFOGEST-standardized digestion protocols (SSF and SGF), biogrape™ ingredient particles underwent enzymatic hydrolysis and exposure to gastric acidity, followed by ultramicrotomy and Transmission Electron Microscopy (TEM), offering high-resolution visual evidence of layered, structurally cohesive encapsulation architectures, preserving morphological integrity and spatial continuity across multiple cross-sections. DPPH antioxidant assays quantitatively assessed post-digestion polyphenol preservation, demonstrating that a significant fraction of bioactives remained bioavailable in the small intestine, achieving retention levels surpassing state-of-the-art encapsulation technologies, yet through a completely natural, self-sustaining process devoid of artificial additives or direct biomaterial manipulation. This in-vitro science-backed mechanism positions biogrape™ as a disruptive, natural-label, and clean-label alternative in the functional ingredient industry, offering a scalable, cost-efficient, and environmentally responsible solution that outperforms conventional encapsulation methods in both efficacy and sustainability. By pioneering this naturally engineered mechanism, POWDER HOUSE redefines bioactive stabilization science, setting a new benchmark in   food technology,   nutraceutical (…)